HDAC1 Substrate Profiling Using Proteomics-Based Substrate Trapping

Substrate-guided proteomics enhances degradome resolution.

Network-based substrate kinetics

Introduction Conclusions References Tables Figures

iTRAQ-based proteomics profiling of Schwann cells before and after peripheral nerve injury

Objective(s): Schwann cells (SCs) have a wide range of applications as seed cells in the treatment of nerve injury during transplantation. However, there has been no report yet on kinds of proteomics changes that occur in Schwann cells before and after peripheral nerve injury.Materials and Methods: Activated Schwann cells (ASCs) and normal Schwann cells (NSCs) were obtained from adult Wistar ra...

GroEL/S substrate specificity based on substrate unfolding propensity.

Phage P22 wild-type (WT) coat protein does not require GroEL/S to fold but temperature-sensitive-folding (tsf) coat proteins need the chaperone complex for correct folding. WT coat protein and all variants absolutely require P22 scaffolding protein, an assembly chaperone, to assemble into precursor structures termed procapsids. Previously, we showed that a global suppressor (su) substitution, T...

Identification of GIT1yCat-1 as a substrate molecule of protein tyrosine phosphatase zyb by the yeast substrate-trapping system

We used a genetic method, the yeast substrate-trapping system, to identify substrates for protein tyrosine phosphatases z (PTPzy RPTPb). This method is based on the yeast two-hybrid system, with two essential modifications: conditional expression of protein tyrosine kinase v-src (active src) to tyrosine-phosphorylate the prey proteins and screening by using a substrate-trap mutant of PTPz (PTPz...